Investigating the therapeutic effect of folic acid conjugated ZnO nanoparticles on human triple negative breast cancer cell line

Shiva Sabzandam; Masoumeh Zahmatkeshan; Moein Adel; Mehrad Mehrdadian; Farzaneh Saliminia; Fariba Esmaeili

doi:10.5937/zasmat2302213S

Autori

Shiva Sabzandam Islamic Azad University, Tehran Medical Sciences, Faculty of Advanced Sciences and Technology, Department of Medical Nanotechnology, Tehran Autor
Masoumeh Zahmatkeshan Iran University of Medical Sciences, Cellular and Molecular Research Center, Tehran, Iran + Iran University of Medical Sciences, Faculty of Advanced Technologies in Medicine, Department of Medical Nanotechnology, Tehran, Iran Autor
Moein Adel Tabriz University of Medical Sciences, Department of Medical Nanotechnology, Tabriz, Iran Autor
Mehrad Mehrdadian Iran University of Medical Sciences, School of Medicine-International Campus, Tehran, Iran Autor
Farzaneh Saliminia Iran University of Medical Sciences, Faculty of Advanced Technologies in Medicine, Department of Medical Nanotechnology, Tehran, Iran Autor
Fariba Esmaeili Tehran University of Medical Sciences, School of Advanced Technologies in Medicine, Department of Medical Nanotechnology, Tehran, Iran Autor

DOI:

https://doi.org/10.5937/zasmat2302213S

Ključne reči:

nanočestica, cink oksid, folna kiselina, trostruko negativni rak dojk

Apstrakt

Trostruko negativan rak dojke (TNBC) čini 15-20% svih invazivnih karcinoma dojke i ima lošu prognozu. ZnO NPs su obećavaju i agensi protiv raka. Štaviše, folatni alfa receptor (FRa) je potencijalni biomarker i terapeutska meta jer je značajno izražen u TNBC. Zbog toga su ZnO i ZnO (F-ZnO) NPs konjugovani sa folnom kiselinom sintetisani sol-gel metodom. NP su karakterisali DLS, zeta potencijal, TEM, FTIR i ICP-MS. FA-ZnO NPs su imali srednji prečnik od 20 ± 2 nm i površinsko naelektrisanje od -15 mV, dok su ZnO NPs imali srednji prečnik od 40 ± 5 nm i površinsko naelektrisanje od -5 mV. MTT test i tripan plavi test, respektivno, korišćeni su za određivanje procenta citotoksičnosti i vitalnosti ZnO i F-ZnO NPs pri različitim koncentracijama od 2, 4, 8, 16, 32, 64 i 128 µg/mL za 12, 24 , 48 i 72 sata (h) na humanoj TNBC ćelijskoj liniji MDAMB-231. Rezultati su pokazali da i ZnO i F-ZnO NPs značajno smanjuju vitalnost ćelija karcinoma na način koji zavisi od doze i vremena (p<0,05). Vrednosti IC50 za FA-ZnO NPs bile su približno 3, 3,74, 4,38, 5,5 puta veće od onih za ZnO NPs u vremenskim tačkama od 12,24, 48 i 72 sata, respektivno. Rezultati sugerišu da F-ZnO NPs imaju potencijal da budu dobra opcija za tretman TNBC-a i da opravdavaju dalja istraživanja.

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