In the present work, effects of microbiological soil properties and phosphomonoesterase activity are presented. The abundances of ammonificators, fungi, actinomycetes, Azotobacter sp., cellulolytic microorganisms, microorganisms that solubilize organic and inorganic P compounds bound to Al, Fe and Ca, have been determined by the dilution method with application of selective nutrient mediums. The first five axes explaines 86% of the total variance. The first axis explains 34.6% of variance and it appears to reflect the processes of microbiological mineralization and immobilization of phosphorus. The second axis explains 25.9% of variance and reflects microbial activity and organic matter accumulation, N biomasses, Azotobacter sp. abundance and acid and alkaline PME activities. Phosphomonoesterase activity in soil depend primarily on soil organic matter and biological factors and can be used as a parameter of plants' uptake of phosphorus, especially in conditions of its low concentration in soil.