Examination of lipophilicity and toxicity of azo dyes derivatives

Abstract

Azo dyes, in addition to showing a wide range of applications in various industries, after releasing into aquatic ecosystems can act as pollutants. The first step in the prediction of possible toxic effects of compounds in the environment includes the estimation of its lipophilicity. Lipophilicity of selected derivatives of azo dyes was examined by thin layer chromatography on a reversed phase (RPTLC18F254s) in t-butanol and N,N-dimethylformamide, as well as by applying appropriate software packages. Relationship between lipophilicity chromatographic parameters (RM0 and m) and values of partition coefficient, log P, as standard measure of lipophilicity was established applying linear regression analysis. Also, relationship between chromatographic parameters (RM0 and m) and computational values of effective concentration, EC50, as a measure of acute toxicity of investigated derivatives for different test organisms was studied. Obtained results show that chromatographic parameters (RM0 and m) could be successfully used for describing lipophilicity (estimation of toxic effects) of examined derivatives of azo dyes.