Nutrition value, as well as macro- and microconstituents of three edible mushrooms (Pleurotus ostreatus, Lentinula edodes, Macrolepiota procera) collected in Serbia were tested. It has been determined that all three mushroom samples were low in energy, fat and carbohydrates, but rich in β-glucans content. The most abundant elements in these three mushrooms were essential marcroelements Ca, K and P, but also there is a significant amount of Li, Se and Zn. On the other hand, toxic elements, such as Pb, As and Cd were also detected in level higher than allowed.