Over the last few decades, nanoparticles of metals have been attracted attention for the wide range of applications in various fields of industry, especially Ag nanoparticles which have been used for their biocidal properties. On the other hand painting is practiced since in ancient times for decoration, but the paint surfaces undergo damage due to natural weathering and the growth and activity of living organisms. Various types of organisms are involved in paint spoilage, such as bacteria, algae and fungi. To prevent paints from biodeterioriation are used biocide substances, which with time have caused environmental and health problems. In this experimental work we aimed to investigate the antifungal efficiency of silver nanoparticles compared to a trade biocide substance sold in our market for paints of indoor environment. A series of microbiological tests were carried out. The results obtained showed that Ag nanoparticles are effective in inhibition of fungal growth.