The purpose of this paper relates to determining the adsorption and recovery degree from activated carbon bags of Sulphorhodamine G Extra (SRG Extra) which can be used as fluorescent tracer in studies of aquatic environments. The effect of the activated carbon is that traces of the used dye are strongly fixed at the surface of the carbon grains. This attribute is used in different water systems studies with fluorescent tracer experiments. Carbon bags are used for the first time in our country in karst system study of Mali me Gropa (2002). Adsorbed tracer can be extracted from carbon bags under the analytical procedures. Based on measurements of SRG Extra fluorescence intensity in standard solutions and extracts we can calculate its adsorption and recovery degree from activated carbon bags. The obtained results prove that extreme low traces of SRG Extra, which cannot be detected directly in the water sample, can be determined in carbon extracts.