Seasonal Assessment of Antibiotic-Resistant Bacteria and Pathogenicity in Rural Tap Water
DOI:
https://doi.org/10.62638/ZasMat1439Abstract
Ordinary people lack reliable drinking water and depend on the usage of tap water for their daily needs. Among them, approximately 20% of families used a water purifier, and 80% used tap water directly. In rural areas, this water is carried to houses through pipelines, which pass through the drainage and are contaminated due to leakage from the pipelines. Mostly, these waters contain antibiotic-resistant bacteria, and direct water use causes a high risk of waterborne disease in humans. In this study, an assessment of antibiotic-resistant bacteria was done during the winter and summer seasons of the tap water. The pathogenicity analysis by bile esculin, D-mannitol test, and their identification by biochemical reactions with the help of Advanced Bacterial Identification Software (ABIS) was carried out. Bacterial colonies were isolated from agar plates through Total Viable Counts (TVC) to determine the specificity of the microbes present in a water sample. Isolates of bacteria were identified based on their morphological, physiological, and biochemical appearances. Susceptibility patterns of isolates were resistant to ofloxacin, cefotaxime, ornidazole, sparfloxacin, co-trimoxazole, cefixime, metronidazole, and norfloxacin through zone of inhibition analysis. This determined the resistance pattern in isolated bacterial strains as per Clinical and Laboratory Standards Institute, 2020, in petriplates after 24 hours. This study is designed to determine the presence of antibiotic-resistant bacteria and their pathogenicity in the collected drinking water, which is alarming for people who insist on demanding additional treatment from different water sources.
Keywords:
Antibiotic-resistant bacteria, Tap water, Antibiotic susceptibilityReferences
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